If you are interested in using a fluorochrome that is not listed here with the Accuri C6, please contact the operator.. Click here to view the instrument manual. It also includes common troubleshooting help and service information. %%EOF 2798 0 obj <>/Filter/FlateDecode/ID[<3B235E56AD4D884590D8BFA25210CF82><756487A21B96FC4983BB2B9C4996548C>]/Index[2774 31]/Info 2773 0 R/Length 99/Prev 1549724/Root 2775 0 R/Size 2805/Type/XRef/W[1 3 1]>>stream Peristaltic Pumps and Luer Locks, Figure 4-5. Many thanks, Matt . Since the C6 fluidics system is non-pressurized, any brand of sample tube that is 12 x 75 mm or smaller can be used. Item is sold AS IS. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open), Changing the Workstation Computer Settings for Windows ® 7, Figure 3-5. Accuri C6 consumables, manuals, etc. !� �XB4��u*��yo7�5�(8.����.�����jo���U߅Ţ?=_��Jk X�3^��=8l�S����6ؽ�6]�w����Wߵ %��S&!bM\w��d�T�����&�B��~��s˟1U�R�l1V&�f�PL�L��K6�c 6�9� BD Accuri C6 Software provides the following features: a. Tabbed views for collection, analysis, and statistics b. This includes microfuge tubes and tubes made of polystyrene and polypropylene. Proper Attachment of Fluidics Lines, Figure 4-1. No. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts. The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. endstream endobj 2778 0 obj <>stream Related Manuals for BD Accuri C6 . BD Accuri C6 Flow Cytometer Instrument Manual Bd Accuri C6 Pdf User Manuals. BD Accuri C6 Instrument Manual (31 pages), Figure 1-1. the BD Accuri C6 Flow Cytometer System, BD Accuri C6 Flow Cytometer Specifications, Figure 3-1. Accuri C6 consumables, manuals, etc. 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Overall very good cosmetic condition. Accuri C6 is a digital PC based bench top analyser, 2 laser system. Click here to view the BD Fluorescence Spectraviewer.. BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. This manual provides information on cytometer requirements and specifications, setup, and maintenance. 2774 0 obj <> endobj ��qq��p�{|�wtxs Such counts are essential in many research and clinical applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring of … Figure 1-1. User's Guides and Manuals. h�b```d``ja`c``�� Ā B,@Q�G����^�M�*O�:.:�c�a��"~��!������Q���ȵ�۵8�1}p�l�����C�+x��L��a:_"�����.��r+��枘�! BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. Standard fluorochrome in use are identical to FACS Calibur. Installation Dialog Box .....80 Figure C-3. Even more colors to choose from with selectable lasers and filter replacement. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. 3. NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. ... Deionised water is recommended in the C6 manual - try this and it may stop blockages. x���MkA�����dI#��b�!�@K =�BH}JK���z,�;��i��w����+���n����s�ج�b;���H The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. �R'�B$K1�#��L�f����2Φ� 5gM�(�,��ycT�}�b��yL]��:y�y�:��~�Kw�s������b\Pע��._H�vtt哂n�mO*���n�s����;;�5����~��;;a��t��~�HX�ÛK `� T���4 d*u�y�Dn�cK�u��A�aZC���_��q�� �#��� ���r��n�Ypm8I_�À�W�"v����,,{�m��c__$�`�r݀��\NiC�{i\ �p=�ƫ�k.x���8�ႃ3��{a�> �6111111111111111��Ǧ�� Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. %PDF-1.6 %���� The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. endstream endobj startxref Came with note that it needs repair. h�bbd```b`` � ��9������"ɸD�NB��!�6�[��f����3>��������L�\�`��X��?�� �YF Please note: It is possible to measure some other fluorochromes using this setup. Core Size/Flow Rate Combinations.....79 Figure C-1. 21. x���M��� `(-X�:��&�̑� r�p�N��S�F?�R4�S�^L=�� I��t�_�n@Q4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_`���=��@J�'� �������(�⍴?�aV(�S��'3*{�����f����߫�!cE��+MH4~������U1Qy+��R�L�Vj��\i@���|����f�M�ZY?�`��bJ�ܠD��Ke��@��T`�e���8�HQ�W�8�7�y����R�T���p�]����ޣ�y.� nW�$�����[�}�k�[j�-J�����[�C�+�gA-�G鳅������g��6v���, ����R�s%�Yu���f�y'��8l���N%. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual . Operate the instrument as described in this Instruction Manual. endstream endobj 2781 0 obj <>stream For Research Use Only. ۟��@��7����O����vƁ`���OW�p�>����8\:�7k������������>q=���!�\�$/-dzCL��)��"�nÒ��2fVo.C��I�aj���~��`��-`v�T�͖(V�` �㆔����rHf ��d)R�7��V�� ר�Gˌ%b��j�Z��.�f>�Z�i-h�`*G-�mA�S,�1&;e,��R�h�Q�4 Lh&4��{�,a��T���.hݓ�t�D�4,�L��J�'�uI�(Y�22��(�t�O�+L�op���z�￠exy����w�c#s����{�u^�(�����H� Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. Available to trained users with online booking. Update Driver Software - Cytometer, Second Dialog Box, Figure 5-4. The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. Manuals and User Guides for BD Accuri C6. y\L }����~*L�l�n}��� 7�x�m����X��gȱ0Z1J50 m��@� �D�1Hğ����H31�m� �� ���' L��� Removing Peristaltic Pump Retainer Clip, Figure 4-6. Services available on the BD Accuri: Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.) h޴S�j�0����2�X���d��BۇM�J��p�l�d�~5����adA�Ci���@�� Press the Power button on the BD Accuri C6 instrument face panel. Automated Preventive Maintenance Notification Window, Figure 4-4. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of … Sliding Luer Lock Fittings Off of Pump Head, Figure 5-2. ?���[��? �! Instructional video on the basics of using BYU's Accuri C6 cytometer. Install, update, and run anti-virus protection software on a regular basis. BD Accuri C6 flow cytometer for the analysis of plant genome sizes. The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. View online or download Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib BD Accuri™ C6 Flow Cytometer Optical Filter Guide . If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. 2804 0 obj <>stream You are viewing an Accuri C6 Flow Cytometer. Accuri™ C6 Plus BD Biosciences. 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. y`�B}�@h�K�v�f�)�*Z�6D�&d'��f�z-C�Zn����R73�nǦ3�-m\�v��tN��ƫ�Zij7��um�zc�&��݌�d7�.� ��L�q����5�H=�Y�pQAں΃��^̑�]#�26m�H-���2'd��5g�Z1GZ;��i�� 9kK:::�;+:8��;:��+::X�;*�,v ����ţ����A(� The instrument will begin a 5 minute auto start cycle. DNA/Cell cycle analysis BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express ™ software and FlowJo ™ software. For a more complete set of directions refer to the BD Accuri C6 Flow Cytometer Instrument Manual . The Accuri C6 cytometer in my lab has some problems, and we need some help. 661085). So the choice should be addressed by the kind of flow experiments you plan to do. BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. Flow cytometry within reach The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. BD Accuri™ C6 Compensation Calculator (Microsoft® Excel®) BD Accuri™ C6 Flow Cytometer Optical Filter Guide . Compare the new data to that provided with the C6 and email the file created in step 20 to TechSupport@AccuriCytometers.com *Steps may be skipped if you purchased your computer from Accuri with your C6. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. (In this example, the file is FCS Express Import Demo.c6.) Update Driver Software - Cytometer, Successfully Updated Your Driver Software, Figure 5-5.The C6 Software Status Message Box - C6 Is Connected and Ready. This item has been powered on, but no further testing. Narrated by Dr. Donald Breakwell Filmed and edited by Gabriel Goulding A unique, non-pressurized, peristaltic pump system drives the fluidics. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. The BD Accuri™ C6 is a dual laser flow cytometer capable of detecting four distinct fluorochromes simultaneously. 0 If the BD Accuri software displays the message Extra Start-up time needed due to cleaning or improper shut down it will take the instrument several more minutes for the instrument to get to the green-light ready state. The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. With minor signs of normal wear and tear. Click here to view the C6 software user guide. Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. endstream endobj 2780 0 obj <>stream Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. Such wear includes scratches, scrapes, and general dirtiness. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. BD Accuri C6: BMSB J538. endstream endobj 2775 0 obj <>/Metadata 476 0 R/Pages 2772 0 R/StructTreeRoot 613 0 R/Type/Catalog>> endobj 2776 0 obj <>/MediaBox[0 0 612 792]/Parent 2772 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2777 0 obj <>stream Accuri C6 flow cytometer fits in any lab and is readily accessible to all researchers—experts and novices alike. Works with current protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers. BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuri™ C6 Software User Guide; BD CSampler™ Accessory Installation Guide; BD CSampler™ Software User Guide The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic … Includes all accessories shown in pictures. �e�I�c~w1���0 ��� endstream endobj 2779 0 obj <>stream User Tracking Installer Icon .....80 Figure C-2. Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. x������ PP9R�P������t�Y�a9D��� ` +9|%��; ��0^��O���-�&+�M�n����I��]�: ���bh��*��f��M�=�>�����}'$�� It also includes … Laboratory Equipment BD CSampler Installation Manual 2 pages. 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